Journal: Materials Today Bio

Article Title: Doxorubicin-loaded PEGylated liposome modified with ANGPT2-specific peptide for integrative glioma-targeted imaging and therapy

doi: 10.1016/j.mtbio.2025.101455

Figure Lengend Snippet: High ANGPT2 expression correlates with GBM progression and poorer prognosis. (A) ANGPT2 mRNA expression in different malignant tumors ( http://gepia.cancer-pku.cn/ ). (B) Comparison of ANGPT2 mRNA expression between normal brain and GBM tissues from TCGA datasets. (C) ANGPT2 mRNA expression in brain tumor at different stages ( http://www.cgga.org.cn/ ), # p < 0.001. (D) ANGPT2 mRNA expression in brain tumor at WHO stage IV in individuals of different gender and age ( http://www.cgga.org.cn/ ). (E) Kaplan-Meier curves estimating overall survival and (F) disease-free survival ( https://smuonco.shinyapps.io/PanCanSurvPlot ) in patients of ANGPT2 mRNA in GBM. Log-rank test, p < 0.001. (G) The observation of ANGPT2 protein expression in different stages of brain tumor tissues. (H) Representative images of ANGPT2 protein expression (a, c, e, and g, magnification 5 × ; b, d, f, and h, magnification 200 × , bar = 50 μm). (I) Positive rate of ANGPT2 in glioma in different stages of brain tumor tissues. (J, K) ANGPT2 expression was analyzed using IB in HA, T98G, U251, and U87-MG cells. ANGPT2 expression was quantified (ANGPT2/β-actin). Normalized ANGPT2 in HA cells was set at 1.0. ∗ p < 0.05, ∗∗ p < 0.01 ( n = 3). (L, M) ANGPT2 levels in membrane proteins were analyzed using IB in T98G, U251, and U87-MG cells. The level of ANGPT2 was quantified (ANGPT2/Na + -K + ATPase). IB, immunoblotting; HA, human astrocyte cell.

Article Snippet: T98G (The human malignant glioma cell lines, no. CL-0583) (Procell Life Science&Technology, Wuhan, China) were cultured in MEM medium with 10 % fetal bovine serum (CM-0583, Procell).

Techniques: Expressing, Comparison, Membrane, Western Blot